ABSTRACT
Objective To investigate the feasibility of macrophages as cell carriers to deliver floate modified oxygen loaded ultrasound contrast agent . Methods The phagocytic activity of macrophages was analyzed by ink phagocytose test , and the expression of folate recepters ( FRs ) on macrophages cell membrane surface was tested by immunofluofluorescence assay . Oxygen/paclitaxel loaded lipid microbubbles( OPLMB) and folate‐targeted OPLMB ( TOPLMB) were synthesized by mechanical shock method and incubated with macrophages in vitro . According to different treatment conditions ,the cells were divided into three groups:group A ( OPLMB) ,group B ( free folic acid + TOPLMB) and group C ( TOPLMB) , the fluorescence intensity of the cells were observed under fluorescence microscope ,and the phagocytic percentage and the phagocytic index of macrophages uptake OPLMB and TOPLMB were observed by bright field microscope . Results The phagocytic percentage of macrophages phagocytose ink was (99 .3 ± 1 .0)% ,FRs was highly expressed on macrophages cultured in vitro . After incubation for 30 minutes ,the fluorescence intensity of group C was significantly higher than those of A and B ,the phagocytic percentage in three groups were (19 .5 ± 0 .2)% ,(21 .0 ± 0 .2)% and (81 .2 ± 10 .0)% respetively . The phagocytic percentage of group C were significantly higher than those in group A and group B ( P <0 .05) . Conclusions Macrophages cultured in vitro possess highly phagocytic activity and these cells highly express FRs ,and can be used as cell carriers to deliver floate modified oxygen loaded multimodality ultrasound contrast agent .
ABSTRACT
Objective To investigate the accuracy of fetal weight estimation by modiefied uterine fundal height.Methods Totally 547 pregnant women admitted to maternity ward of the Second Affiliated Hospital of Chongqing Medical University from December 1,2013 to April 30,2014 with childbirth were chosen as the subject.The modified uterine fundal height were measured by the uterus inclination and modiefied fundal height admeasuring apparatus,normal uterine fundal height and and abdomen circumfer-ence were measured by tape.The fetal weight was estimated by utilizing modified uterine fundal height.Results The modified fun-dal height was(31.7±2.30)cm,which was significantly higher than that normal fundal height(34.7 ± 2.46 )cm(t = 54.25,P <0.01).The accuracy rate of modified uterine fundal height for fetal weight estimation with empirical formulas,Yuan′s,Zhuo′s,Lin′s,Zen′s,Gu′s were 68.4%,69.7%,59.4%,67.8%,69.3%,78.4%,respectively.The accuracy rate of normal uterine fundal height for fetal weight estimation with the same formulas were 53.4%,34.4%,54.3%,46.1%,38.8%,45.9%,respectively.The accuracy rate of modiefied uterine fundal height for fetal weight estimation with empirical formulas,Yuan′s,Lin′s,Zen′s,Gu′s were significantly higher than that of utilizing normal uterine fundal height estimation with the same formulas(P <0.01).Conclusion Modified uterine fundal height has better accuracy in estimating fetal weight,it has a important clinical significance.
ABSTRACT
Objective To investigate the feasibility and parameters for macrophage uptake oxygen and paclitaxel loaded lipid microbubbles (OPLMBs) in vitro.Methods OPLMBs were synthesized by mechanical vibration method and incubated with macrophages in different conditions in vitro.The ratio of macrophages uptake OPLMBs was observed by bright field microscope.Results The ratio of macrophages uptake OPLMBs was closely related to the size,concentration as well as the incubation time of OPLMBs with the macrophage.The ratio of macrophages uptake OPLMBs had a reverse relationship with the size of microbubbles.The highest rate of macrophages uptake OPLMBs was (18.9 ± 0.69)％ when the ratio of macrophages to microbubbles was 1 to 10,the time for macrophages co-incubation with microbubbles was 30 min,which was the suitable condition for macrophages uptake OPLMBs.Conclusions OPLMBs can be phagocytized successfully by macrophages in vitro.The size,concentration as well as the incubation time of OPLMBs with the macrophages are important parameters contributed to the efficiency of macrophage phagocytosis.
ABSTRACT
Objective The purpose of this study was to compare chemerin and CRP levels between women with and without ear‐ly‐onset preeclampsia based on maternal body mass index (BMI) .Methods 45 patients with early onset preeclampsia (gestation week is less than 34 weeks) ,30 normal pregnancy women were enrolled in the study .The serums levels of chemerin and CRP were measured in these pregnancy women .Results The chemerin [(379 .18 ± 52 .09)ng/L ,(481 .27 ± 63 .12)ng/L] and CRP [(7 .59 ± 1 .41)mg/L ,(11 .74 ± 1 .61)mg/L] levels in MPE and SPE were significantly higher than those [(316 .08 ± 51 .90)ng/L ,(3 .47 ± 1 .27)mg/L]in normal pregnancy women(P<0 .05) .While the chemerin(424 .64 ± 46 .61)ng/L and CRP (8 .32 ± 3 .82)mg/L levels increased with maternal BMI in patients overweight and obese women levels compared with normal weight women [(331 .66 ± 51 .77)ng/L ,(5 .93 ± 2 .95)mg/L] ,the difference are significant(P<0 .05) .The correlation analysis showed that the chemerin were positively correlated with BMI ,systolic blood pressure ,diastolic blood pressure ,triglycerides ,and CRP were positively correlated with BMI .Conclusion The expression of chemerin and CRP were associated with severity of preeclampsia and BMI .
ABSTRACT
<p><b>OBJECTIVE</b>To observe the clinical effect of low-intensity ultrasound in promoting uterine involution following cesarean section.</p><p><b>METHODS</b>A total of 122 women undergoing cesarean section were randomly selected and divided into low-intensity ultrasound treatment group (67 cases) and control group (55 cases). The women in the treatment group received daily low-intensity ultrasound treatment for 30 min 24 h after the delivery for 3 consecutive days, and the control group had no particular treatments. The descent of the uterine fundus and cessation of lochia was observed in the two groups.</p><p><b>RESULTS</b>The treatment group showed obviously greater descent of the uterine fundus with a higher rate of cessation of vaginal bleeding than the control group at 30 days postpartum. The ratio of incomplete uterine involution was significantly lower in the treatment group than in the control group.</p><p><b>CONCLUSION</b>Low-intensity ultrasound is effective in promoting uterine contraction and uterine involution following cesarean section.</p>
Subject(s)
Adult , Female , Humans , Pregnancy , Young Adult , Cesarean Section , Rehabilitation , Postpartum Period , Ultrasonic Therapy , Methods , Ultrasonography , Uterus , Diagnostic ImagingABSTRACT
The cephalic dystocia education is an important part of obstetric education. According to current educational environment, the teaching method of multimedia combined with problem-based learning is conducive to resolving the dilemma of dystocia education. Dystocia education is designed to follow the cases as the main line and the problem as a guide, with the full integration of theory and practice. This teaching model has been recognized by the majority of foreign students, and it is worth recommending.
ABSTRACT
This article gives an introduction to the background and process of founding online teaching platform by department of obstetrics and gynecology of the hospital in teaching practice.It presents a brief discussion on the application of professional online teaching platform to specific teaching and administration.It also shows the content construction of online platform and the need of students for it.Besides,it explores the important role of online platform in future education.
ABSTRACT
From the current situations and problems on bed side teaching of gynaecology and obstetrics,the paper discusses the strategy and the development trend of gynaecology and obstetrics bed side teaching in the future.
ABSTRACT
Objectives:To investigate the causes and effect factors of postpartum hemorrhage(PPH)in order to prevent PPH and decrease the mortality rate of gravida and parturient.Methods:Retrospective analysis was done on the 74 cases of PPH from Jan 2002 to Jan 2006 in our hospital.Results:The incidence rate of PPH during 4 years was 2.33%(74/3178).The causes were uterine inertia(43.24%),abnormal placenta(39.19%),injury of birth canal(6.67%),and dysfunction of blood coagulation(1.35%).There was significant difference among delivery style,gestational weeks,multiple pregnancy,and complications.(P
ABSTRACT
Objective: To investigate the anxiety and depression in pregnant with diabetes.Methods:A survry was conducted among 60 pregnant women with diabetes and 188 normal pregnant women in the Second Afflicated Hospital of Chongqing Medical science from 2001~2003 using self-rating anxiety scal(SAS) and self-rating depression scale(SDS).Results: The average SAS in pregnant women with diabetes was 55.5?4.59,significantly higher than that in the normal group(31.9?3.0,P